Fig 1: Immunohistochemical staining of co-culture groups after 28 days of incubation. (A) Positive PTH staining was observed as brown cytoplasmic color in thyroid-parathyroid co-culture organoids in dif medium. (B) TTF1 staining was observed negative in thyroid-parathyroid co-culture organoids in dif medium. (C) TTF1 staining was observed positive with brown nuclear color (red arrows) in thyroid-parathyroid co-culture organoids in st medium. Scale bars represent (A) 20 µm and (B–C) 50 µm. dif = differentiation, PTH = parathormone, st = standard, TTF1 = thyroid transcription factor 1.
Fig 2: Immunofluorescence staining of proteins in thyroid cells using confocal microscopy before differentiation protocol. (A) TSHR (membrane) and (B) TTF1 (nuclear), Thyroid cells stained in green for all proteins and counterstained in blue for nuclei. The cytoskeleton was stained with phalloidin red to observe cell morphology. Objective 40 × original magnification, scale bars represent 50 µm. TSHR = thyroid stimulating hormone receptor, TTF1 = thyroid transcription factor 1.
Fig 3: Whole animal St18 KO produces a specific loss of MGE lineage GPe projection neurons.a Quantification of PV+ neurons (Unpaired t-test). N = 6 WT and 9 St18 (-/-). b Quantification of Nkx2-1+ neurons (Unpaired t-test). N = 4 WT and 4 St18 (-/-). c Quantification of Er81+ neurons (Unpaired t-test). N = 4 WT and 5 St18 (-/-). Quantification of Nkx2-1/PV double-positive (Unpaired t-test). N = 4WT and 4 St18 (-/-). d Quantification of Npas1+ neurons (Unpaired t-test). N = 4 WT and 6 St18 (-/-). e Quantification of FoxP2+ neurons (Unpaired t-test). N = 4 WT and 4 St18 (-/-). f Quantification of s100b + glial cells (Unpaired t-test). N = 4 WT and 4 St18 (-/-). All data assessed by two-sided Data are presented as mean +/- SEM. Source data are provided as a Source data file. Scale bar represents 500 microns.
Fig 4: Conditional St18 KO produces a specific loss of MGE lineage GPe projection neurons.a Quantification of PV + neurons (Unpaired t-test). N = 19 WT and 18 St18 cKO. b Quantification of Nkx2-1+ neurons (Unpaired t-test). N = 11 WT and 12 St18 cKO. c Quantification of Er81+ neurons (Unpaired t-test). N = 20 WT and 18 St18 cKO. d Schematic representation of adult mouse brain in the parasagittal plane showing axonal projections of GPe prototypic neurons (orange) targeting the STN (blue). e WT and St18 cKO STN (outlined in blue) with MGE lineage whole cell labeled fate map (Nkx2-1Cre; Ai9). Quantification of axonal innervation area (Unpaired t-test). N = 9 WT and 27 St18 cKO. f WT and St18 cKO STN with (left) MGE lineage synaptophysin labeled fate map (Nkx2-1Cre; Ai34). tdTomato, (right) 3D segmented puncta quantified for puncta density and puncta size (Unpaired t-test). N = 27 WT and 12 St18 cKO. Data are presented as mean +/- SEM. Source data are provided as a Source data file. Scale bars: a–c, e 500 microns; f 100 microns.
Fig 5: The co-expression of TTF-1 and P40 in the cells of the cancer-like lesion in 18-month-old LXRαβ-/- mouse lung. TTF-1[SP141] and P40 staining on serial sections showed co-expression of TTF-1 and P40 in the cells of peripheral bronchial basal layer adjacent to the lesion (red arrows), TTF-1 positive and P40 negative in the cells of luminal layer (black arrows) (A1, B1). Insert pictures in A1 and B1 (TTF-1[EPR8190-6] and P63 staining respectively) showed basal cells are both TTF-1 positive and P63 positive (red arrow), luminal cells were TTF-1 positive and P63 negative (black arrow). TTF-1[SP141] and P40 were co-expressed in the cells of the lesion (A2, B2). Insert picture in A2 was TTF-1[EPR8190-6] staining and insert picture in B2 was P63 staining. Double immunofluorescence staining for TTF-1 (red), P40 (green) and DAPI (blue) confirmed the co-expression of TTF-1 and P40 in the cells (white arrows) (C). (Scale bars in A and B, 200 μm and 50 μm. Scale bars in C, 30 μm.)
Supplier Page from Abcam for Anti-TTF1 antibody [SP141]